Table 1 Primer sequences for RT-qPCR
From: PGM5-AS1 impairs miR-587-mediated GDF10 inhibition and abrogates progression of prostate cancer
Gene | Forward sequence | Reverse sequence |
|---|---|---|
PGM5-AS1 | 5′-GACTATGTTGTGAGCCTGCG-3′ | 5′-AAAAGGGGAGGGGCAATACA-3′ |
miR-587 | 5′-CCAGGCAAGAGAGAGTTGCTG-3′ | 5′-AGTCACAGGTGCAGACACATT-3′ |
GDF10 | 5′-GGACTTTGACGAGAAGACGATG-3′ | 5′-TCTTAGGCATGGGGAACTCAC-3′ |
U6 | 5′-CTCGCTTCGGCAGCACA-3′ | 5′-AACGCTTCACGAATTTGCGT-3′ |
β-actin | 5′-CCTGGCACCCAGCACAAT-3′ | 5′-GCCGATCCACACGGAGTACT-3′ |