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Fig. 6 | Journal of Translational Medicine

Fig. 6

From: PGM5-AS1 impairs miR-587-mediated GDF10 inhibition and abrogates progression of prostate cancer

Fig. 6

PGM5-AS1 overexpression suppresses prostatic tumorigenesis in nude mice. a The relative expression of PGM5-AS1, miR-587 and GDF10 in the xenograft prostatic tumor after transduction through RT-qPCR assay. b The quantitative analysis for volume change of the xenograft prostatic tumors in nude mice measured by a vernier caliper. c The representative images of the xenograft prostatic tumor in nude mice. d The quantitative analysis for weight of the xenograft prostatic tumor in nude mice. e The immunohistochemical staining of Ki67 expression in tumor tissues (×200). f The quantitative analysis of Ki67 expression in tumor tissues. g TUNEL staining of cell apoptosis of tumor tissues (×200). h The quantitative analysis of cell apoptosis of tumor tissues. i The protein bands of expression of cleaved caspase-3, Bax and Bcl-2 in tumor tissues through Western blot analysis. j LDH release determined by ELISA. n = 5. *p < 0.05 vs. the oe-NC group (nude mice injected with cells treated with oe-NC). The measurement data were summarized as mean ± standard deviation. Comparisons among multiple groups were conducted by one-way ANOVA with Tukey’s post hoc test. Statistical analysis in relation to time-based measurements within each group was realized using repeated measures ANOVA, followed by a Bonferroni’s post-hoc test. The experiment was repeated three times independently

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