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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: PGM5-AS1 impairs miR-587-mediated GDF10 inhibition and abrogates progression of prostate cancer

Fig. 5

PGM5-AS1 inhibits malignant progression of PCa cells via upregulation of GDF10 by binding to miR-587. a The relative expression of PGM5-AS1, miR-587 and GDF10 in transfected PCa cells as evaluated by RT-qPCR; *p < 0.05 vs. the blank group (untreated PCa cells). b The quantitative analysis of cell proliferation in transfected PC-3 cells as measured by EdU assay. c The quantitative analysis of expression of Ki67 and PCNA in transfected PC-3 cells through Western blot analysis. d The quantitative analysis of cell colony formation in transfected PC-3 cells. e The quantitative analysis of cell apoptosis in transfected PC-3 cells detected by flow cytometric analysis. f The protein bands of expression of cleaved caspase-3, Bax, RIP3, cyclophilinA and Bcl-2 in transfected PC-3 cells through Western blot analysis. g LDH release determined by ELISA. *p < 0.05 vs. the oe-PGM5-AS1 + mimic-NC group (PCa cells overexpressing PGM5-AS1 treated with mimic-NC). #p < 0.05 vs. the oe-PGM5-AS1 + sh-NC group (PCa cells overexpressing PGM5-AS1 treated with sh-NC). The measurement data are summarized as mean ± standard error. Comparison between two groups was conducted by independent sample t-test. The experiment was repeated three times independently

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