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Fig. 4 | Journal of Translational Medicine

Fig. 4

From: PGM5-AS1 impairs miR-587-mediated GDF10 inhibition and abrogates progression of prostate cancer

Fig. 4

miR-587 upregulation accelerates proliferation, colony formation and impedes apoptosis of PC-3 cell line by targeting GDF10. a The prediction of target genes of miR-587. b GDF10 expression in GSE30994 dataset. c The predictive analysis on online website (https://www.targetscan.org/) for binding sites between miR-587 and GDF10. d The dual-luciferase reporter assay to verify miR-587 binding to GDF10. e RT-qPCR detection of relative expression of GDF10, PGM5-AS1 and miR-587 in PCa cells treated with miR-587 mimic. f The protein bands of expression of GDF10 protein in PCa cells treated with miR-587 mimic through Western blot analysis. g The quantitative analysis of expression of GDF10 protein in PCa cells treated with miR-587 mimic through Western blot analysis. h The relative expression of miR-587, GDF10 and PGM5-AS1 in transfected PCa cells as evaluated by RT-qPCR; *p < 0.05 vs. the blank group (untreated PCa cells). i The quantitative analysis of cell proliferation in transfected PC-3 cells as measured by EdU assay. j The protein expressions of Ki67 and PCNA in transfected PC-3 cells through Western blot analysis. k The quantitative analysis of cell colony formation in transfected PC-3 cells. l The quantitative analysis of cell apoptosis in transfected PC-3 cells detected by flow cytometric analysis. m The quantitative analysis of expression of cleaved caspase-3, Bax, RIP3, cyclophilinA and Bcl-2 in transfected PC-3 cells through Western blot analysis. n LDH release determined by ELISA. *p < 0.05 vs. the mimic-NC group (PCa cells treated with mimic-NC) or the inhibitor-NC + sh-NC group (PCa cells treated with inhibitor-NC + sh-NC). #p < 0.05 vs. the miR-587 inhibitor + sh-NC group (PCa cells treated with miR-587 inhibitor + sh-NC). The measurement data are summarized as mean ± standard error. Comparison between two groups was conducted by independent sample t-test. Comparisons among multiple groups were conducted by one-way ANOVA with Tukey’s post hoc test. The experiment was repeated three times independently

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