Fig. 3
From: PGM5-AS1 impairs miR-587-mediated GDF10 inhibition and abrogates progression of prostate cancer
PGM5-AS1 competitively binds to miR-587. a The subcellular localization of PGM5-AS1 as evaluated by RNA-FISH (×400). b miR-587 that binds to PGM5-AS1 was screened out by DIANA Tools database. c The verification of the binding relationship between PGM5-AS1 and miR-587 through dual-luciferase activity detection. *p < 0.05 vs. the mimic-NC group. d RIP assay to verify the binding relationship between PGM5-AS1 and miR-587. *p < 0.05 vs. the IgG group. e RNA pull-down analysis of the binding relationship between PGM5-AS1 and miR-587. *p < 0.05 vs. the NC-bio group. f miR-587, PGM5-AS1 and GDF10 expression in PGM5-AS1 overexpressing cells measured by means of RT-qPCR assay. The measurement data are summarized as mean ± standard error. Comparison between two groups was conducted by independent sample t-test. Comparisons among multiple groups were conducted by one-way ANOVA with Tukey’s post hoc test. The experiment was repeated three times independently