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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: In vivo vaccination with cell line-derived whole tumor lysates: neoantigen quality, not quantity matters

Fig. 5

IFNγ ELISpot and tumor microenvironment. a Reactivity of peripheral blood leukocytes against Mlh1−/− target cells as assessed during vaccination and at the experimental endpoint. The number of IFNγ secreting cells/5 × 104 effector cells was determined after overnight co-incubation and quantification on an ELISpot reader as described in material and methods. *p < 0.05; **p < 0.01; ***p < 0.001 one-way ANOVA (Bonferroni multiple comparison). b Immunofluorescence was done on 4 µM slides stained with mAbs as stated in “Material and methods” section. Cell nuclei were stained with DAPI. Control tumors were highly infiltrated with CD11b+ granulocytes and F4/80+ macrophages. The infiltration pattern changed dependent on the vaccine. Analyses were done on a laser scanning microscope (Zeiss) using 20× objectives

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