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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: Allogeneic administration of human umbilical cord-derived mesenchymal stem/stromal cells for bronchopulmonary dysplasia: preliminary outcomes in four Vietnamese infants

Fig. 1

Characterization of hUC-MSC sources for allogeneic administration to patients with severe BPD. a hUC-MSCs were obtained from a healthy donor after written informed consent was given. The morphology of hUC-MSCs (P3) expanded in xeno- and serum-free culture medium was spindle-shaped, and the cells were adherent, forming a monolayer in 2D culture. b The cells maintained a normal karyotype after 6 passages in culture in vitro, with a population doubling time of 28 ± 1.3 h. c The hUC-MSCs exhibited colony-forming features (140 ± 15 CFU/1000 cells, mean ± SEM, n = 3) and d were able to differentiate into three lineages. e Assessment of MSC markers using flow cytometry confirmed the expression of MSC-positive markers (CD73, CD90, and CD105 > 98%) and less than 2% negative markers. Scale bar: 100 µm

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