Fig. 6

Biochemical measurements of proteins and serum angiotensin-converting enzyme activities. A marked decrease of the PGC1α levels could be detected in DOX-treated animals compared to CON. a FoxO1 levels appeared to be lower in the D-CON group compared to CON, however, this change did not reach statistical significance. b No significant changes were recognisable in the pACC/ACC levels between the groups. c Serum ACE measurements revealed a significantly lower ACE activity in the ACEI group compared to CON, and a numerically less different, but significantly decreased value in the BB group. d A robust decrease in the serum ACE₂ levels of the DOX-exposed animals could be seen compared to healthy controls. e n = number of animals per group (1–4 bands/animal for Western immunoblots, 2 measurements/animal for ACE and ACE2 activities); Statistics: Wilcoxon’s rank-sum test; numbers are p values. ACC = acetyl coenzyme A carboxylase, AU = optical density in arbitrary units, FoxO1 = Forkhead box protein O1, P–ACC = phosphorylated ACC, PGC1α = peroxisome proliferator-activated receptor-gamma coactivator 1 alpha