Fig. 2

Characterization of Extracellular Vesicles. (a) Morphology of isolated EVs from ME/CFS was confirmed by transmission electron microscopy (a representative image is shown; scale bars: 200 nm and 500 nm) and (b) Western blot analysis of isolated EVs from healthy controls (CTRL) and ME/CFS subjects as representative samples. Thirty μg of protein was loaded in each lane and probed with specific antibodies to EV protein markers. Cytochrome C (mitochondrial marker) was used as negative control for EV and positive for PBMCs