Fig. 3

LPS treated Mo-DCs can be efficiently generated in FEP bags and TCPS plates. Flow cytometry was performed before culture (Start) and after 2, 7 and 9 days of differentiation and maturation culture on FEP or TCPS surfaces. Shown are Tukey's Box-and-whisker plots. ns: no statistically significant differences for both paired (n ≥ 4 donors for all surface markers at all time points except n = 3 for CD86 at day 7) and unpaired (n = 14 donors at day 0; n = 4 to 10 donors at other time points except n = 3 for CD86 day 7) comparisons