TY - JOUR AU - Bastien, Jean-Philippe AU - Fekete, Natalie AU - Beland, Ariane V. AU - Lachambre, Marie-Paule AU - Laforte, Veronique AU - Juncker, David AU - Dave, Vibhuti AU - Roy, Denis-Claude AU - Hoesli, Corinne A. PY - 2020 DA - 2020/10/09 TI - Closing the system: production of viral antigen-presenting dendritic cells eliciting specific CD8+ T cell activation in fluorinated ethylene propylene cell culture bags JO - Journal of Translational Medicine SP - 383 VL - 18 IS - 1 AB - A major obstacle to anti-viral and -tumor cell vaccination and T cell immunotherapy is the ability to produce dendritic cells (DCs) in a suitable clinical setting. It is imperative to develop closed cell culture systems to accelerate the translation of promising DC-based cell therapy products to the clinic. The objective of this study was to investigate whether viral antigen-loaded monocyte-derived DCs (Mo-DCs) capable of eliciting specific T cell activation can be manufactured in fluorinated ethylene propylene (FEP) bags. SN - 1479-5876 UR - https://doi.org/10.1186/s12967-020-02543-1 DO - 10.1186/s12967-020-02543-1 ID - Bastien2020 ER -