Fig. 4
From: Novel single-domain antibodies against the EGFR domain III epitope exhibit the anti-tumor effect
Effect of the five anti-EGFR sdAbs on inhibiting cancer cell migration by cell scratch assay. Cells were incubated for 24 h with the five anti-EGFR sdAbs (aEG1B4, aEG2C7, aEG2E12, aEG4D9 and aEG6B2) at different concentrations (0, 25, 50 and 100 µg/ml). Two sdAbs (aVE201 and aHer2-13C1) were included as negative controls. Images were photographed after 0 and 24 h following making a scratch on a cell monolayer. The scratch widths were measured by Image-Pro Plus 6.0 software. a, b A549 cells; c, d MCF-7 cells; e, f DU145 cells. Data are shown as mean ± S.D (n = 5). *P < 0.05 and **P < 0.01 vs. the respective control (0 µg/ml)