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Fig. 2 | Journal of Translational Medicine

Fig. 2

From: A persistently replicating SARS-CoV-2 variant derived from an asymptomatic individual

Fig. 2

Persistence of SARS-CoV-2 GZ69 in Vero E6 cells. a CPE induced by SARS-CoV-2 AP66 and SARS-CoV-2 GZ69 in Vero E6 at P0 (72 h p.i.) (Original magnification 10x). b SARS-CoV-2 RNA copy number was calculated in cell supernatants collected at P0 by qRT-PCR. Values represent the S gene copies/ml mean ± SD of the triplicate. c Bright field of persistent infected SARS-CoV-2 GZ69 at P4 (Original magnification 10x). d Immunofluorescence of Vero E6 cells persistently infected with SARS-CoV-2 GZ69 at P4. Images display SARS-CoV-2 signals in green and cell nuclei in blue (Scale Bar 20 µm). e SARS-CoV-2 RNA copy number was calculated in cell supernatants collected prior to cell subculture by qRT-PCR. Times of supernatant collection are indicated with a T. Values represent the S gene copies/ml mean ± SD of the triplicate. NI not infected

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