Fig. 5

Functional assay of RB-340-1F double transduced cells. a Numbers of CAR + T cells existing in co-culture during 3 days killing assay against Fadu/PD-L1. b Numbers of Fadu/PD-L1 cells existing in co-culture during 3 days killing assay with non-transduced T cells (NT), RB-340-1F double transduced cells treated with or without BX795. c PD-1 positive percentage in double transduced T cells during 3 days killing assay against Fadu/PD-L1. Nosg groups are T cells transduced with LdCK-GFP vector and CAR-TEV-mCherry control vector without carrying any sgRNA sequence. PD1sg groups are RB-340-1F that contains LdCK-GFP and CAR-TEV-mCherry carrying PD-1 sgRNA sequence. d The concentration of secreted IL2, TNFα, and IFNγ in killing assay supernatant collected on day 1. All above results are the averages of triplicate wells. They are representative of five experiments done with T cells from two donors. e The average results of cytokine release from five experiments. To emphasize the fold change, cytokine concentrations in BX795 non-treated group are all normalized to 1. *p < 0.05; ns, not significant