Fig. 5

Transmission electron micrograph of the EVCs. a Shows the ultrastructure of the EVCs. Due to different sizes, EVC population is heterogeneous and contains exosome and microvesicles. Internalization potential of the extracellular vesicles exposed to osteoblasts (b). The red particle in the osteoblast indicates internalized ECVs. Also, flow cytometry of a representative sample of ECVs (c) shows that they were positive for CD₆₃, CD₉ and CD₈₁