Fig. 2

The sections of decellularized scaffolds (a, c) and intact bone (b, d) stained with Hoechst and H&E. Both staining confirm cell and nuclei depletion. Also, the graph (e) compares DNA content of decellularized scaffold and the intact bone. Scanning electron microphotographs revealed scaffold porosity, ultrastructure preservation and orientation of the collagen fibers of decellularized scaffold (f) and intact bone (g). * Significant difference with control group (P < 0.05)