Fig. 5

Chronic loss of STAG2 subtly alters global transcriptional programmes. a RNA-SEQ density plots of Log2 gene counts in the Δ STAG2 cells, compared to the STAG2-WT cells. Changes greater than or less than 1.5-fold (−/ + Log2 0.585) are indicated in green (decreased) and red (increased). b Volcano plot displaying the 3149 significantly altered (adj p < 0.05) genes between the WT and ΔSTAG2 cells. c Deregulated Haematological developmental processes gene network identified through Ingenuity Pathway Analysis (IPA) as altered in ΔSTAG2 cells compared to STAG2-WT cells. Genes whose expression was increased or decreased in Δ STAG2 cells are highlighted in red and green respectively. d ChIP-Seq derived average signal density plots depicting binding profiles for STAG1 and STAG2 centred on transcription start sites (TSSs) (−2.5–2.5 kb) of all coding genes. e ChIP-Seq derived average signal density plots depicting binding profiles for STAG1 and STAG2 centred on transcription start sites (TSSs) (-2.5 – 2.5 kb) of genes whose expression was deregulated more than 1.5 fold in the ΔSTAG2 cells