Fig. 1

Generation of an isogenic model of chronic STAG2 loss. a Sanger sequencing chromatograms from OCI-AML3 cells in which the single copy of STAG2 (X chromosome) was targeted for editing with CRISPR/Cas9, creating a deletion in exon 20, generating a frameshift mutation (11 bp deletion, 1 bp insertion) resulting in apremature stop codon, 5 codons downstream. The resultant STAG2 mutant cell line is labelled ΔSTAG2. Chromatogram from unedited STAG2-WT cells is shown for comparison. b Normalized RNA-seq gene count levels highlighting the significant reduction in STAG2 mRNA expression (gene counts include all known STAG2 isoforms) in ΔSTAG2 cells compared to STAG2-WT cells. c Normalized RNA-seq gene count levels highlighting the mRNA expression levels of members of the cohesin complex in STAG2-WT cells and ΔSTAG2 cells. d Representative Western Blots demonstrating expression of cohesin complex members in STAG2-WT cells and ΔSTAG2 cells