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Fig. 5 | Journal of Translational Medicine

Fig. 5

From: MiR-199a-3p/5p participated in TGF-β and EGF induced EMT by targeting DUSP5/MAP3K11 in pterygium

Fig. 5

MiR-199a-3p and miR-199a-5p target DUSP5 and MAP3K11 respectively. a, b Venn diagram of miR-199a-3p and miR-199a-5p target genes prediction results from 4 databases. c, d Putative miR-199a-3p and miR-199a-5p binding sites in the 3′UTR of DUSP5 and MAP3K11, and the designed mutation sites. e, f A luciferase reporter plasmid containing wild type or mutant type were co-transfected with relevant mimic, mimic NC, inhibitor and inhibitor NC into HCEs. Luciferase activity was normalized to Renilla luciferase activity. Of note, MAP3K11 3′UTR had 2 binding sites withmiR-199a-5p, so 3 kinds of mutants were construct, including mutant at the first site, mutant at the second site and mutant at both sites (**p < 0.01, ***p < 0.001, ****p < 0.0001). gj After HCEs were transfected with miR-199a-3p/miR-199a-5p mimic, mimic NC, miR-199a-3p/miR-199a-5p inhibitor and inhibitor NC, the variations of DUSP5 and MAP3K11 expression were analyzed with qRT-PCR and western blot (*p < 0.05, **p < 0.01, ***p < 0.001)

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