Fig. 4
From: Identification of MiR-211-5p as a tumor suppressor by targeting ACSL4 in Hepatocellular Carcinoma
MiR-211-5p binds with 3′-UTR of ACSL4 and negatively modulates ACSL4 expression. a The 3′-UTR of ACSL4 contains a putative binding site for miR-211-5p and a mutation of the 3′-UTR of ACSL4 was designed. b, c The luciferase assay indicated that overexpression of miR-211-5p by mimic significantly reduced the luciferase activity of ACSL4-WT, while this difference could not be observed in the luciferase activity of ACSL4-MUT in LM3 (b) and SK Hep-1 (c) cells. d The results of qRT-PCR showed the relative ACSL4 mRNA expression level of LM3 and SK Hep-1 cells in control group, transfecting miR-211-5p mimics group, and transfecting miR-211-5p inhibitor group. e The protein expression level of ACSL4 and β-Actin in LM3 and SK Hep-1 cells. *p < 0.05, **p < 0.01, ***p < 0.001