Expression and clinical significance of LAG-3, FGL1, PD-L1 and CD8+T cells in hepatocellular carcinoma using multiplex quantitative analysis

Table 1 Relationship between immune cell markers and clinicopathological features (n = 143)

Variable	LAG-3			CD8			PD-L1 TC			PD-L1 IC			FGL1 TC			FGL1 IC
Variable	Low	High	P	Low	High	P	Negative	Positive	P	Negative	Positive	P	Negative	Positive	P	Negative	Positive	P
Patients	83	60		53	90		91	52		60	83		84	59		113	30
Age(years)			0.137			0.512			0.366			0.166			0.313			0.008
> 50	32	16		16	32		58	37		36	59		53	42		69	26
≤ 50	51	44		37	58		33	15		24	24		31	17		44	4
Gender			0.192			0.163			0.182			0.428			0.827			0.890
Male	13	5		4	14		77	48		54	71		73	52		99	26
Female	70	55		49	76		14	4		6	12		11	7		14	4
HbsAg			0.627			0.595			0.642			0.608			0.936			0.540
Negative	12	7		6	13		13	6		9	10		11	8		14	5
Positive	71	53		47	77		78	46		51	73		73	51		99	25
Cirrhosis			0.077			0.565			0.475			0.172			0.002			0.284
No	46	42		31	57		54	34		33	55		43	45		67	21
Yes	37	18		22	33		37	18		27	28		41	14		46	9
AFP(ng/ml)			0.189			0.656			0.340			0.696			0.245			0.813
≤ 20	41	23		25	39		38	26		28	36		41	23		50	14
> 20	42	37		28	51		53	26		32	47		43	36		63	16
Vascular invasion			0.231			0.783			1.000			0.179			1.000			1.000
Absent	79	53		48	84		84	48		58	74		78	54		102	28
Present	4	7		5	6		7	4		2	9		6	5		11	2
Tumor size (cm)			0.652			0.145			0.008			0.501			0.141			0.102
≤ 5	58	44		34	68		58	44		41	61		56	46		77	25
> 5	25	16		19	22		33	8		19	22		28	13		36	5
Tumor number			0.381			0.329			0.899			0.975			0.618			0.103
Single	71	48		42	77		76	43		50	69		71	48		97	22
Multiple	12	12		11	13		15	9		10	14		13	11		16	8
Tumor differentiation			0.489			0.289			0.515			0.452			0.196			0.675
I-II	75	52		49	78		82	45		54	73		77	50		101	26
III	8	8		4	12		9	7		6	10		7	9		12	4
BCLC stage			0.668			0.071			0.054			0.701			0.643			0.321
0 + A	50	34		26	58		48	36		36	48		48	36		64	20
B + C	33	26		27	32		43	16		24	35		36	23		49	10

P < 0.05 was considered statistically significant, Pearson χ² tests
HBsAg hepatitis B surface antigen, AFP αfetoprotein, BCLC Barcelona Clinic Liver Cancer, TC tumor cells, IC immune cells

ISSN: 1479-5876