Fig. 3
Effects of ExoC6-cer on the expressions of miR-29b and Akt pathway in HUVECs. a The expression of miR-29b in HUVECs was quantified by using real-time PCR after co-cultured with ExoC6-cer for 48 h. b qPCR showed the expressions of Akt3, PI3K and VEGFA in OPM2 after ExoC6-cer treatment (left-hand panels). Western blots showed the expressions of Akt3, P-AKT, PI3K and VEGFA in OPM2 after ExoC6-cer treatment (right-hand panel). β-Actin is used as normalization control for the total lysate. The data shown were representatives of three independent experiments that gave similar results. c qPCR showed the expressions of Akt3, PI3K and VEGFA in RPMI-8226 after ExoC6-cer treatment (left-hand panels). Western blots showed the expressions of Akt3, P-AKT, PI3K and VEGFA in RPMI-8226 after ExoC6-cer treatment (right-hand panel). β-Actin is used as normalization control for the total lysate. All the experiments were performed in triplicates. The data shown were representatives of three independent experiments that gave similar results. (*P < 0.05)