Fig. 7

rHMGB1 preconditioning inhibits AM pyroptosis via the Keap1/Nrf2/HO-1 pathway in LIRI. a Isolated AM counts in BALF. b LDH release from isolated AMs in BALF. c Representative results of flow cytometry assessing macrophage pyroptosis: F4/80⁺ cells were gated and analyzed for fluorescently labeled active caspase (FLICA) and propidium iodide (PI). d Quantitative analysis of F4/80⁺FLICA⁺PI⁺ cells. e Representative immunolabelling images for GSDMD protein from isolated AMs in BALF. f GSDMD levels in isolated AMs. (*p < 0.05, compared to control group, ^#p < 0.05, compared to I/R group, ^{^}p < 0.05, comparison between rHMGB1 + I/R and brusatol + rHMGB1 + I/R group)