Fig. 5

Keap1/Nrf2/HO-1 pathway inhibition alleviates the protective effects of rHMGB1 preconditioning in LIRI. a Western blot of nuclear Keap1 in lung tissues. b Western blot of nuclear Nrf2 in lung tissues. c Western blot of cytosolic HO-1 in lung tissues. d Morphological changes across groups observed using H&E staining. Magnification, ×200. e Lung injury scoring. f Wet/dry ratios in lung tissues. g IL-1β abundance in lung tissues. h IL-6 abundance in lung tissues. i NF-κB abundance in lung tissues. (*p < 0.05, compared to control group, ^#p < 0.05, compared to I/R group, ^{^}p < 0.05, comparison between rHMGB1 + I/R and brusatol + rHMGB1 + I/R group)