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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: 32A9, a novel human antibody for designing an immunotoxin and CAR-T cells against glypican-3 in hepatocellular carcinoma

Fig. 1

GPC3 expression in HCC patient tissue and HCC cell lines. a. Immunohistochemistry staining of GPC3 in HCC patient tumor tissues and normal liver tissues. Scale bar: 100 μm. b Western blot to detect GPC3 expression in HCC cells. c Flow cytometry analysis to detect GPC3 cell surface expression in the indicated cells. Filled curve: Isotype control staining. Open curve: anti-GPC3 staining. A431 and A431-GPC3 are set up as GPC3-negative cell line control and GPC3-positive cell line control for GPC3 staining, respectively

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