Fig. 5

Imaging the spinal cord and frontal cortex ASO distribution and pharmacology. Representative images from MALAT1 ISH (left three columns) and ASO IHC (two columns on the right) in representative animals from the naïve animal, the low (0.8 mL) volume no wrap, and high (2.4 mL) volume no wrap groups. Images are arranged top to bottom with the proximal (lumber spinal cord) to distal (frontal cortex) areas from the injection site. In the spinal cord pictures, white matter is designated by WM and gray matter by GM. The tissue concentrations in ug/g tissue measured by LC–MS in the adjacent tissues are displayed in the ASO IHC pictures. The MALAT1 RNA expression values (percent of naïve control) as measured by PCR from the adjacent spinal cord tissues are written on the ASO treated MALAT1 ISH pictures. Horizontal black bars are scale bars representative of 100 µm