Fig. 1
a Imaging rejection criteria for poor technical injections in the 64Cu-DOTA experiment. The PET/CT scan on the left is indicative of a successful IT injection where the radioisotope is mostly contained in the neuraxial compartments. The PET/CT scan on the right illustrates an unsuccessful IT injection where a high radioisotope signal can be seen in the kidneys, ureters and bladder. Sites of injection of the 64Cu-DOTA are indicated by arrows. PET images were smoothed with a 2.5 mm Gaussian filter and scaled from 0 to 2% ID/g indicated by the color scale bar. b Graph of the ASO tissue concentrations in µg/g tissue in the lumbar spinal cords of all the animals. Animals with lumbar spinal cord tissue concentrations below 3 µg/g tissue were considered probable missed injections due to technical reasons. There were two animals in both low volume (0.8 mL) groups and one animal in each high dose volume (2.4 mL) group that had lumbar spinal cord concentrations below 3 µg/g