Fig. 3
Differentially methylated probes in gastric cardiac IM. a Volcano plot of probe-level methylation in IM versus normal. The plot shows the relationship between magnitude of difference in β values (Δβ values; x-axis) and adjusted p values (negative log10 transformed adjusted p values; y-axis). Each dot represents a single probe. The cutoff of adjusted p = 0.01 and 20% in methylation difference (|Δβ| = 0.2) are marked by horizontal and vertical dashed lines, respectively. Red and blue dots denote hyper- and hypomethylated DMPs, respectively. The percentages of hyper- and hypomethylated DMPs are displayed (bottom). Source data are provided in Additional file 3: Table S2. b Bar charts showing the distribution of DMPs in relation to gene region (left panel) and CpG island region (right panel). The distribution of CpG probes was as follows: the total probes in the 850K array available for analysis (grey; n = 733,015 probes), hypermethylated DMPs (red; n = 21,528 probes), and hypomethylated DMPs (blue; n = 16,709 probes) according to gene region (left panel) and CpG island region (right panel). P values were calculated by Chi squared tests (****p < 0.0001). Source data are provided in Additional file 4: Table S3