Fig. 3

The expression and intracellular localization of wild-type and mutated FGF10. Relative mRNA expression of wild-type and variants of FGF10 in HCM (a) and HEK 293T (c), respectively (n = 3). GAPDH was used as an internal control. Western blot analysis and density quantitation in HCM (b) and HEK 293T (d) transfected with the blank vector, wild-type, and mutated FGF10. GAPDH and β-actin were used as an internal control in HCM and HEK293T, respectively (n = 3). e Immunofluorescence staining of wild-type and variants of FGF10. Images represented here were obtained from 3 biological replicates. Scale bar 20 μm