Table 1 Clinical characteristics of childhood acute leukaemia patients involved in discovery and validation cohorts

CNS+ patient group

 P1

ALL, late-onset Rel^b

lymphoid cells with unambiguous blasts (CNS-2)

d0

d0, d15

d0, d15, d30

d0, d15, d30

 P2

ALL, de novo^c

86 WBCs; high amount of blasts (CNS-3)

d0, d15

d0, d15, d32, d45

d37

d0, d37

 P3

ALL, de novo^a

1 unambiguous blast, 2 suspicious cells (CNS-2)

d0

d0, d15, d33

d0, d15, d33

d0, d15, d33

 P4

ALL, de novo^a

4 WBCs/μl with blasts (CNS-2)

d0, d15

‒

‒

‒

 P5

ALL, de novo^a

18 lymphoid cells (mainly blasts), 20 Gumprecht shadows (CNS-2)

‒

d0, d15

d0, d15

d0, d15

 P6

ALL, de novo^a

1 unambiguous blast (CNS-2)

‒

d0, d15, d33

d0, d15, d33

d0, d15, d33

 P7

MPAL, de novo^a

some cells, 70% blast (CNS-2)

‒

d15, d33

d0, d15, d33

d0, d15, d33

 P8

AML, de novo^d

53 cells; 8% blast (CNS-2)

‒

d0, d15

d0

d0, d15

 P9

AML, de novo^d

7 WBCs/μl; numerous blasts (CNS-3)

‒

d0, d28

d0, d28

d0, d28

 P10

ALL, early-onset Rel^b

43 WBCs, mostly blasts (CNS-3)

‒

d0

d0

‒

 P11

ALL, de novo^a

nucleated cells, 70% blast (CNS-2)

‒

d0, d15

‒

‒

 P12

ALL, de novo^a

35 lymphoid cells with 10% blast (CNS-2)

‒

d0, d15, d26, d40

‒

‒

CNS⁻ patient group

 P13

ALL, de novo^a

1 cell/μl without blasts (CNS-1)

d0

‒

‒

‒

 P14

ALL, de novo^a

without cells (CNS-1)

d0

‒

‒

‒

 P15

ALL, de novo^a

without cells (CNS-1)

d0

‒

‒

‒

 P16

ALL, de novo^a

1 cell/μl without blasts (CNS-1)

d0

‒

‒

‒

 P17

ALL, early-onset Rel^b

squamous cells, 2 lymphocyte, 1 Gumprecht shadow (CNS-1)

‒

d0, d15

d0, d15, d37

d0, d15, d37

 P18

ALL, early-onset Rel^b

without cells (CNS-1)

‒

d0, d15

d0, d15

d0, d15

 P19

ALL, de novo^a

without cells (CNS-1)

‒

d0, d15, d33

d0, d15, d33

d0, d15, d33

 P20

ALL, de novo^a

only a few RBCs (CNS-1)

‒

d0, d15

d0, d15, d33

d0, d15, d33

 P21

ALL, de novo^a

2 cells without blasts (CNS-1)

‒

d0, d15, d33

d0, d15, d33

d0, d15, d33

 P22

MPAL, de novo^a

a few squamous cells on cytospin (CNS-1)

‒

d15, d38

d0, d15, d38

d0, d15, d38

 P23

AML, de novo^d

some RBCs without blasts (CNS-1)

‒

d0

d0, d23

d0, d15, d23

 P24

AML, de novo^d

1 segmented, 2 band neutrophil granulocytes (CNS-1)

‒

d0, d28

d0, d15, d28

d28

 P25

ALL, de novo^a

1 segment, 8 WBC (CNS-1)

‒

d0

d0

‒

 P26

ALL, de novo^a

without cells (CNS-1)

‒

d0

‒

‒

 P27

ALL, de novo^a

without cells (CNS-1)

‒

d0

‒

‒

 P28

ALL, de novo^a

4 segmented neutrophil cells, 3 lymphocytes, 1 monocyte, 1 Gumprecht shadow, but no blasts (CNS-1)

‒

d0

‒

‒

 P29

ALL, de novo^a

without cells (CNS-1)

‒

d0

‒

‒