Fig. 4

Gene expression between donor and xenografted liver metastases. a, b Differential expressed genes between donor and matched xenografted liver metastases samples of patient #1(A) and #2 (B) Top 10 up-regulated genes and down-regulated genes in patient #1 and #2 were listed in tables above. Multiple genes with similar patterns of expression (fold change < 4) were also listed in both matched samples, such as TP53, KRAS, NRAS, EGFR, ERBB2, APC, CTNNB1, AXIN2, SMAD4 and CDK2. c Pearson’s correlation between donor liver metastases and xenografted liver metastases samples based on the RNA-seq data of driver genes. Pearson’s correlation coefficient in patient #1(r = 0.82, p-value = 0.001) and #2 (r = 0.99, p-value = 0.001) were showed. LM, liver metastases; PDOX-LM, patient-derived organoids-based xenografted liver metastases