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Fig. 1 | Journal of Translational Medicine

Fig. 1

From: A novel patient-derived organoids-based xenografts model for preclinical drug response testing in patients with colorectal liver metastases

Fig. 1

Establishment of the PDOX-LM model. a Preparation of primary tumor derived organoids for splenic injection. Representative images of primary tumor-derived organoids under the light microscope (above) and the morphologic feature of organoids were observed by hematoxylin and eosin (H&E) staining (below), Bar = 200 μm. b Representative live confocal images of a Lgr5, E-cadherin dual positive organoids cultures in Matrigel (BD science). Blue represents nuclear, which is staining by Hoechst 33342 (left, above). Green represents cell membrane of lgr5-Positive organoids, which indicated existence of the stem cell property (right, above). Red represents cell membrane of the E-cadherin-positive organoids, which indicated the cell in organoids were rooted from epithelial source (left, below). Representative image of Lgr5 and E-cadherin dual positive organoids were also showed (right, below). Bar = 150 μm. c Xenografted liver metastases by a splenic injection of primary tumor derived organoids. Representative pictures of splenic xenografts and xenografted liver metastases of patient #1(left, above) and #2(right above) in the mice models. White tumor xenografts were labeled(arrowhead). In picture of patient #1, only 1 xenografts in murine spleen and 2 metastatic xenografts in the murine liver were detected under macrography. In picture of patient #2, 2 xenografts in murine spleen and 4 metastatic xenografts in the murine liver were detected under macrography. Based on organoids derived from localized CRC, the xenografts were only observed in the spleen but not in the liver in mice models. Representative picture of splenic xenografts of patient #7 (left, below) and #8 (right, below) were showed. d Human CEA in murine blood. CEA,a serum biomarker expressed from CRC cells. Quantitative ELISA with human specific antibodies. e Human CEA were compared between the mCRC group (n = 24) and CRC group (n = 24). NOTE: *p value ≤ 0.05 and **p-value ≤ 0.01 when compared with CRC group; f Human CEA were also compared among the ‘No xenografts’ group (n = 8), the ‘Only splenic xenografts’ group (n = 25) and the ‘Both splenic and liver xenografts’ group (n = 15). NOTE: *p-value ≤ 0.05, **p-value ≤ 0.01, and ***p-value ≤ 0.001, respectively, when compared with ‘No xenografts’ group; g Body weight of mice after splenic xenotransplantation. After a mean period of 6 weeks after splenic xenotransplantation, a decrease in body weight of 21 mice (21/24, 87.5%) were observed

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