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Table 2 List of antibodies used for immunocytochemistry or flow cytometry

From: Human induced pluripotent stem cell line banking for the production of rare blood type erythrocytes

Antibodies used for immunocytochemistry
Marker type Antibody Company, Cat#
Embryonic stem cell markers Rabbit anti-human OCT4 Abcam, Cat# ab109884
Rabbit anti-human SOX2
Rabbit anti-human NANOG
Mouse anti-human TRA-1-60
Mouse anti-human SSEA4
Secondary antibodies Alexa Fluor 594 donkey anti-rabbit IgG Life Technologies, Cat# A-21207
Alexa Fluor 488 goat anti-mouse IgG Life Technologies, Cat# A-11001
Antibodies used for flow cytometry
Embryonic stem cell markers PE mouse anti-human TRA-1-60 BD Biosciences, Cat# 560193
FITC mouse anti-human SSEA4 BioLegend, Cat# 330410
Differentiation markers FITC mouse anti-human CD34 BD Biosciences, Cat# 555821
APC mouse anti-human CD43 BD Biosciences, Cat# 560198
PE mouse anti-human CD235a BD Biosciences, Cat# 555570
APC mouse anti-human CD71 BD Biosciences, Cat# 551374
Isotype control FITC mouse IgG BioLegend, Cat# 401306
PE mouse IgM BD Biosciences, Cat# 555584
APC mouse IgG BD Biosciences, Cat# 555751
  1. APC, allophycocyanin; Cat, catalogue; FITC, fluorescein isothiocyanate; PE, phycoerythrin