Table 2 List of antibodies used for immunocytochemistry or flow cytometry
From: Human induced pluripotent stem cell line banking for the production of rare blood type erythrocytes
Antibodies used for immunocytochemistry | ||
|---|---|---|
Marker type | Antibody | Company, Cat# |
Embryonic stem cell markers | Rabbit anti-human OCT4 | Abcam, Cat# ab109884 |
Rabbit anti-human SOX2 | ||
Rabbit anti-human NANOG | ||
Mouse anti-human TRA-1-60 | ||
Mouse anti-human SSEA4 | ||
Secondary antibodies | Alexa Fluor 594 donkey anti-rabbit IgG | Life Technologies, Cat# A-21207 |
Alexa Fluor 488 goat anti-mouse IgG | Life Technologies, Cat# A-11001 | |
Antibodies used for flow cytometry | ||
|---|---|---|
Embryonic stem cell markers | PE mouse anti-human TRA-1-60 | BD Biosciences, Cat# 560193 |
FITC mouse anti-human SSEA4 | BioLegend, Cat# 330410 | |
Differentiation markers | FITC mouse anti-human CD34 | BD Biosciences, Cat# 555821 |
APC mouse anti-human CD43 | BD Biosciences, Cat# 560198 | |
PE mouse anti-human CD235a | BD Biosciences, Cat# 555570 | |
APC mouse anti-human CD71 | BD Biosciences, Cat# 551374 | |
Isotype control | FITC mouse IgG | BioLegend, Cat# 401306 |
PE mouse IgM | BD Biosciences, Cat# 555584 | |
APC mouse IgG | BD Biosciences, Cat# 555751 | |