Fig. 3
From: Human induced pluripotent stem cell line banking for the production of rare blood type erythrocytes
Counting erthyroblast cells to determine the date for transfection: a separated PB-MNCs were enriched with cytokines adequate for promoting erythroid progenitors. Typically, erythroblast population exceeded 80% on expansion day 7. b flow cytometry analysis of 7-days enriched erythroid progenitors presenting CD235a and CD71 antigens. c On erythroblast expansion day 7, if the observed erythroblast population (blue arrow) was less than 80%, transfection was performed after extending the expansion step for 2–3 days in the same conditions