Fig. 1From: Human induced pluripotent stem cell line banking for the production of rare blood type erythrocytesThe scheme of the production of human induced pluripotent stem cells from peripheral blood. After isolating the PB-MNCs using Ficoll-Paque, cells are enriched in erythroid expansion medium for 7 days to stimulate the growth of erythroid precursors. When erythroid progenitor cells reach 80% of the total PB-MNCs, cells are transfected with reprogramming vectors (Oct4, Sox2, Lin28, Klf4, and L-Myc)Back to article page