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Table 3 Fcγ- and C3b-receptor mediated erythrophagocytosis with MSA controls

From: Complement activating ABO anti-A IgM/IgG act synergistically to cause erythrophagocytosis: implications among minor ABO incompatible transfusions

Immunoglobulin

Complement source

Phagocytosis (%)

BRAD3 + IgM Anti-A

–

1

BRAD3 + IgM Anti-A

Pooled fresh Group A serum

30

BRAD5 + IgM Anti-A

–

2

BRAD5 + IgM Anti-A

Pooled fresh Group A serum

51

IgM Anti-A

–

1

IgM Anti-A

Pooled fresh Group A serum

3

  1. RBCs coated with complement C3b only showed < 5% phagocytosis. A significant increase in monocyte phagocytosis was observed upon complement C3b activation relative to RBCs sensitized with BRAD3 or BRAD5 only. Dilutions used = 1:10000 BRAD3, 1:800 BRAD5, 1:6000 murine monoclonal anti-A. Group A plasma was used in the place of pooled Group A serum as a negative control
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Journal of Translational Medicine

ISSN: 1479-5876

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