Fig. 5
From: Development and optimization of orthotopic liver metastasis xenograft mouse models in uveal melanoma
Long term monitoring of hepatic tumor with IVIS image was performed in splenic injection with splenectomy model. NSG mice were injected UM001-tdTomato cells (2.0, 1.0, 0.5, and 0.25x106 cells/mouse, 5 mice of each group) into spleen, then spleen was removed 15Â min after injection. IVIS image has been taken every week and mice will be euthanized using C02 exposure followed by cervical dislocation if they show 20% weight loss or severe weakness. a 2.0x106 cells/mouse, b 1.0x106 cells/mouse, c 0.5x106 cells/mouse and d 0.25x106 cells/mouse. The fluorescent intensity between the anterior portion and the posterior portion of the liver in the e 1.0 and f 0.25x106 cells injected mice were compared