TY - JOUR AU - Sugase, Takahito AU - Lam, Bao Q. AU - Danielson, Meggie AU - Terai, Mizue AU - Aplin, Andrew E. AU - Gutkind, J. Silvio AU - Sato, Takami PY - 2020 DA - 2020/05/20 TI - Development and optimization of orthotopic liver metastasis xenograft mouse models in uveal melanoma JO - Journal of Translational Medicine SP - 208 VL - 18 IS - 1 AB - Patients with metastatic uveal melanoma (MUM) in the liver usually die within 1 year. The development of new treatments for MUM has been limited by the lack of diverse MUM cell lines and appropriate animal models. We previously reported that orthotopic xenograft mouse models established by direct injection of MUM cells into the liver were useful for the analysis associated with tumor microenvironment in the liver. However, considering that patients with UM metastasize to the liver hematogenously, direct liver injection model might not be suitable for investigation on various mechanisms of liver metastasis. Here, we aim to establish new orthotopic xenograft models via hematogenous dissemination of tumor cells to the liver, and to compare their characteristics with the hepatic injection model. We also determine if hepatic tumors could be effectively monitored with non-invasive live imaging. SN - 1479-5876 UR - https://doi.org/10.1186/s12967-020-02377-x DO - 10.1186/s12967-020-02377-x ID - Sugase2020 ER -