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Table 2 Dilution assay

From: A novel NGS-based microsatellite instability (MSI) status classifier with 9 loci for colorectal cancer patients

Dilution, %

Tumor purity, %

MSI score

MSI status

100

32

0.87

MSI-H

50

16

0.46

MSI-H

33

10.6

0.26

MSS

20

6.4

0.20

MSS

100

67

1.26

MSI-H

50

33.5

0.67

MSI-H

33

22.3

0.42

MSI-H

20

13.4

0.28

MSS

  1. Two MSI-H samples with tumor contents of 32% and 67% were selected for gradient dilution experiments by diluting tumor DNAs with their matched normal DNAs. The mixtures were tested for MSI status by USCI-msi
  2. MSI-H microsatellite instability-high, MSS microsatellite stability