Fig. 1

Schematic illustration of the selection pipeline (a–c) for microsatellite loci. 363 mononucleotide loci in our panel region were selected (a) and used for detecting MSI status. The 363 microsatellite loci were then sorted in descending order by the mean instability score calculated by MANTIS in MSI-H and MSS cases (b). The overlap of the top 50 loci in MSI-H cases and the bottom 50 loci in MSS cases was chosen for training USCI-msi (c)