Fig. 4

Detection of Intracellular effector molecules in CD8⁺dT cells and their activation after coculturing with autologous trophoblasts. a Representative FACS plots (Left) and percentages (Right) of the expression of intracellular granzyme B and perforin in total CD8⁺pT cells and CD8⁺dT cells; b IFN-γ, IL-4 expression in total CD8⁺pT cells and CD8⁺dT cells stimulated for 6 h with PMA/Ionomycin (1 μg/mL); c Lymphocytes from the decidua and PBMC were cultured separately with autologous trophoblasts for up to 24 h and were then tested for expression of CD69 and IFN-γ. Graphs show the data of four to ten samples in each group; a paired t-test was used to calculate the p-values, *p = < 0.05, ***p = < 0.005, ****p = < 0.0001