Fig. 3

Count and hemolysis parameter correlation of circulating blood vesicles. Vesicle counts (events/µL) in plasma samples from patients undergoing major cardiac surgery with cardiopulmonary bypass (CPB) with (n = 9; CytoSorb; black triangles) or without (n = 9; control; gray circles) hemoadsorption. Samples were analyzed using flow cytometry. Data are presented as mean ± standard deviation (a). Scanning electron microscopy was performed to analyze a hemoadsorption column directly after use. Respective pictures are shown in ×400 and ×5000 magnification (b). Correlation analysis was performed to assess the link between vesicle counts, hemolysis and infection parameters. The color represents the respective rho-value and the presence of circles indicate a significant p-value < 0.05 (c)