Fig. 1

Flow cytometric setup and gating strategy. Green fluorescent silica beads (1000 nm) were used to define the side scatter properties and the microvesicle (MV) gate was set right underneath the silica bead population (left panel). Using an unlabeled plasma sample, the scatter and gate settings were validated as seen in the right panel without any trigger. All events above the MV-gate were assumed to be larger than 1000 nm and were separately analyzed as apoptotic bodies (ABs). a Next, the fluorescence trigger was set for the APC-channel (Annexin V (AnnV)) using an AnnV-stained plasma sample without calcium, preventing calcium-dependent labelling of AnnV (left Panel). The fluorescence trigger was set and validated using an AnnV-labelled plasma sample (right panel; b). The gating strategy of CD41 + MVs (platelet-derived MVs; left panel) and CD235 + (erythrocyte-derived MVs; right panel) (c)