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Fig. 6 | Journal of Translational Medicine

Fig. 6

From: Beneficial impact of epigallocatechingallate on LDL-C through PCSK9/LDLR pathway by blocking HNF1α and activating FoxO3a

Fig. 6

EGCG reduces PCSK9 but increases LDLR expression and activity were associated with FoxO3a and HNF1α in HepG2 cells. The nuclear extracts from with or without EGCG (25 μM) –treated HepG2 cells. a The effects of EGCG on the nuclear HNF1α and FoxO3a expression by Western blot analysis, the normalized intensity of nuclear HNF1α and FoxO3a protein, HDAC2 was used as the loading control. b HepG2 cells was transfected with siRNA negative control (si-Control) or stealth siRNA for the knockdown of HNF1α or FoxO3a (si-FoxO3a), the level of PCSK9 was determined by western blot, GAPDH was used as the loading control. c Representative fluorescence microscopy images of cell‐associated Dil‐LDL (red), Hoechst‐stained nuclei (blue) and the overlay (upper). Fluorescence of isopropanol‐extracted Dil (520–570 nm, normalized to the cell protein) (lower). Data are presented as mean ± SEM (N = 3). *p < 0.05, **p < 0.01 compared with control. ##p < 0.01 compared with EGCG group

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