Table 1 The primer sequence used in RNA based PCR
GENE | Forward primer | Reverse primer |
|---|---|---|
SOX2 | ATGGGTTCGGTGGTCAAGTC | CGCTCTGGTAGTGCTGGGACA |
CD133 | AGTCGGAAACTGGCAGATAGC | GGTAGTGTTGTACTGGGCCAAT |
GAPDH | CTGGGCTACACTGAGCACC | AAGTGGTCGTTGAGGGCAATG |
CDK4 | ATGGCTACCTCTCGATATGAGC | CATTGGGGACTCTCACACTCT |
CDK6 | TCTTCATTCACACCGAGTAGTGC | TGAGGTTAGAGCCATCTGGAAA |
CYCLIND1 | CAATGACCCCGCACGATTTC | CATGGAGGGCGGATTGGAA |
EZH2 | AATCAGAGTACATGCGACTGAGA | GCTGTATCCTTCGCTGTTTCC |
KDM6A | TTCCTCGGAAGGTGCTATTCA | GAGGCTGGTTGCAGGATTCA |
KDM6B | CGCTGCCTCACCCATATCC | ATCCGCGACCTCTGAACTCT |