Fig. 3

miR-1271 interacts with PES1 and negatively regulates PES1 expression. a Regulatory miRNAs of PES1 predicted using the mirDIP, miRDB, miRSearch, and TargetScan databases; four ellipses refer to the prediction results in four databases respectively and the intersection among these ellipses indicating overlapped results. b Binding site between miR-1271 and PES1 predicted using the TargetScan. c Luciferase activity in response to co-transfection of miR-1271 mimic and NC mimic or PES1-3′UTR-Wt and PES1-3′UTR-Mut, as detected by dual-luciferase reporter gene assay. d Expression of miR-1271 and PES1 following treatment with miR-1271 mimic, miR-1271 inhibitor or their respective NCs, as determined using RT-qPCR. e Protein expression of PES1 in response to miR-1271 mimic, miR-1271 inhibitor or their respective NCs tested by Western blot analysis. *p < 005 vs. treatment with NC mimic; #p < 0.05 vs. treatment with NC inhibitor. Data obtained from three independent experiments were presented as mean ± standard deviation and analyzed using independent sample t-test between two groups and using one-way ANOVA among multiple groups