Fig. 6

Effect of IL-24 treatment on HK-2 cells. Presence of IL-20RB (red) was determined by immunofluorescence staining on HK-2 cells (a). Cell nuclei were counterstained with Hoechst 33342 (blue). Cell viability was determined by MTT (b) and LDH (c) assays. The mRNA expression of TGFB1 (d) PDGFB (g) CTGF (j) after IL-24 treatment (100 ng/ml) was measured by real-time RT-PCR in comparison with GAPDH as internal control. The protein level of TGF-β1 (e, f) PDGF-B (h, i) after IL-24 treatment (100 ng/ml) was measured by flow cytometry (g, h). The protein level of CTGF (k, l) after IL-24 treatment (100 ng/ml) was measured by Western blot analysis in comparison with GAPDH as internal control (l and Additional file 1: Figure S4d.) Values were expressed as mean ± SD. n = 5–6 in each group; *p < 0.05 vs. control (Mann–Whitney U-test). Scale bar: 50 µm (a)