Fig. 4

HMGB1 impaired the anti-inflammation efficacy of MSCs in vivo. a Renal mRNA levels of TNF-α, IL-1β and IL-6 were analyzed by qPCR. b Serum levels of TNF-α, IL-1β and IL-6 were determined by ELISA. c Intact kidneys were decapsulated, diced, and digested for single cell suspensions. Renal single cells were then incubated with anti-mouse CD3, CD4 and CD8 antibodies and subsequently analyzed by flow cytometry. Data were shown as mean ± SD (n = 6 mice per group). *P < 0.05