Fig. 1

Increased level of HMGB1 in the serum and kidney following IRI. Mice subjected to bilateral renal pedicle clamping for 15, 30 and 45 min were sacrificed at day 1 after reperfusion: Renal mRNA a and protein levels b of HMGB1 were analyzed by qPCR and western blot analysis separately; c Serum levels of creatinine (Scr) and blood urea nitrogen (BUN) were detected; d HMGB1 protein, Scr and BUN expressed as a percentage of those parameters for sham groups (n = 6 mice per group). Mice subjected to bilateral renal pedicle clamping for 30 min were sacrificed at the indicated time point: Renal mRNA e and protein levels f of HMGB1 were analyzed by qPCR and western blot analysis separately; g Serum levels of Scr and BUN were detected; h HMGB1 protein, Scr and BUN expressed as a percentage of those parameters for sham groups (n = 6 mice per group). i Serum level of HMGB1 was determined 24 h after reperfusion with bilateral renal pedicle clamping for 30 min (n = 5 mice per group). j Mice were subjected to bilateral renal pedicle clamping for 30 min and then received recombinant HMGB, A box, B box (1 mg/kg body weight) or vehicle PBS by intraperitoneal injection immediately after reperfusion. These mice were sacrificed at day 1 for detection of kidney function (n = 6 mice per group). Data were shown as mean ± SD. *P < 0.05, **P < 0.01