Fig. 3

Quantification of IHC staining. a Digitalised image analysis approach of IHC staining, a showcase of Melan-A IHC. Melan-A staining of the showcase (P2) sample in 3D hanging drop cell culture. The positive staining area (red) recognised by colour thresholding using the “Lab” colour space was annotated in yellow; non-staining area (blue/grey) was annotated in purple, scale bar = 200 µm. b Quantification of expressed histological markers for proliferation, diagnosis and representative cellular components in original MSE, 2D and 3D hanging drop cell cultures. The ratio (percentage) of positive pixels to the total number of pixels of each marker was presented